robotic benchtop microarrayer omnigrid micro  (Digilab Inc)

Journal: PLoS ONE

Article Title: Development of a High-Throughput Candida albicans Biofilm Chip

doi: 10.1371/journal.pone.0019036

Figure Lengend Snippet: ( A ) Correlation of fluorescence intensities of spots obtained after FUN 1 staining with the number of viable cells present in each spot. The fluorescence intensities were measured using a microarray scanner and the values are expressed as RFUs. ( B ) Fluorescence intensity of nano-biofilms on Ca BChip stained with FUN 1. Values represent mean and standard deviations of multiple independent biofilms formed in each of 9 different blocks of the chip.

Article Snippet: The suspension containing yeast cells, collagen and media was printed (50 nL per spot) on the functionalized PSMA-coated glass slides using a microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA).

Techniques: Fluorescence, Staining, Microarray

Journal: PLoS ONE

Article Title: Development of a High-Throughput Candida albicans Biofilm Chip

doi: 10.1371/journal.pone.0019036

Figure Lengend Snippet: ( A ) Schematic of attachment of collagen-encapsulated C. albicans biofilms to modified glass slides. Glass slides modified with APTES were coated with PSMA and a suspension of C. albicans yeast cells in microbiological media and collagen as encapsulating material was spotted onto the glass slides. ( B ) The optimal concentrations of growth media, inocula, collagen and PSMA that supported maximum biofilm yield in spots that also attached robustly to substrate were obtained from a factorial design study. ( C ) A picture of the high-thoughput Ca BChip, printed using a robotic microarrayer and containing 768 spots on PSMA-coated slides. Each hemispherical spot is 50 nL in volume, approximately 700 µm in diameter, and with a 1.2 mm separation between spots. Also shown is a fluorescent picture of fungal biofilms formed on the chip and stained with FUN 1, taken with a microarray scanner, alongside a close-up image of nano-biofilms.

Article Snippet: The suspension containing yeast cells, collagen and media was printed (50 nL per spot) on the functionalized PSMA-coated glass slides using a microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA).

Techniques: Modification, Staining, Microarray

Journal: PLoS ONE

Article Title: Development of a High-Throughput Candida albicans Biofilm Chip

doi: 10.1371/journal.pone.0019036

Figure Lengend Snippet: ( A ) Light microscopy: a series of microphotographs showing the formation of nano-biofilms over time upon incubation at 37°C after initial printing using the robotic microarrayer. Magnification is ×100 for all panels. ( B ) Growth kinetics of C. albicans biofilm formation in Ca BChip as determined using FUN 1 staining and a microarray reader. Cells were printed on the chips, incubated at 37°C and fluorescence readings were taken over a 48 hour period. Results are expressed as arbitrary Relative Fluorescence Units (RFUs).

Article Snippet: The suspension containing yeast cells, collagen and media was printed (50 nL per spot) on the functionalized PSMA-coated glass slides using a microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA).

Techniques: Light Microscopy, Incubation, Staining, Microarray, Fluorescence